Regulatory

Part:BBa_J100200:Design

Designed by: Malcolm Campbell   Group: Campbell M Lab   (2014-10-13)


tClone tetA v2


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1138
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1284
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1310
    Illegal NgoMIV site found at 1678
    Illegal NgoMIV site found at 1838
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 899
    Illegal BsaI.rc site found at 42


Design Notes

Need to screen for internal BsaI sites. Flanked by BioBrick endings and having this synthesized as two G blocks so that we can clone this faster and cheaper than as an entire gene.


Source

This was designed in the Campbell and Eckdahl labs and is related to plasmid J119137 (pClone Red).

References